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Fluorescence Cube

 

A fluorescence cube (also called a fluorescence filter cube or filter set cube) is an essential optical component used in fluorescence microscopes. It is a compact housing that integrates the optical filters and dichroic mirror required for fluorescence imaging. These cubes are inserted into the microscope’s fluorescence turret, allowing quick switching between different fluorophores.

Key Features of High-Quality Fluorescence Cubes

  • High transmission efficiency in both excitation and emission bands.
  • Deep blocking outside the passbands to prevent bleed-through.
  • Hard-coated interference filters for durability and spectral stability.
  • Rigid cube body with precision alignment of optical elements.
  • Zero pixel shift design for accurate multi-color imaging.
  • Compatibility with various fluorophores (DAPI, GFP, FITC, Rhodamine, Cy3, Cy5, etc.).
    • Code Filter Excitation Wavelength Dichroic Mirror Wavelength Emission Wavelength
    R1-CFP-WB-450 R1-CFP 420-445 450 460-510
    R1-GFP-WB-495 R1-GFP 455-485 495 500-545
    R1-GFP-WB-505 Blue (FITC) 465-20 505 515
    R1-YFP-WB-510 R1-YFP 490-500 510 520-560
    R1-UV-WB-400 R1-UV 330-380 400 420
    R1-UV-WB-410 R1-UV 340-390 410 420
    R2-UV-NB-400 R2-UV 365/10 400 400
    R2-DAPI-WB-420 DAPI(UV) 370/12 420 475-90
    R3-UV-WB-400 R3-UV 330-380 400 435
    R3-V-WB-430 R3-V 380-420 430 460
    R1-V-WB-430 R1-V 380-420 430 450
    R1-BV-WB-448 R1-BV 448/23 465 472
    R1-BV-WB-455 R1-BV 400-440 455 470
    R2-BV-WB-455 R2-BV 400-410 455 455
    R2-BV-NB-455 R2-BV 435/10 455 470
    R1-B-SB-500 R1-B 450-480 500 515
    R1-B-SB-505 R1-B 420-490 505 520
    R2-B-SB-515 Blue (FITC) 420-485 505 515
    R2-B-SB-520 Blue (FITC) 420-495 505 520-550
    R2-B-WB-500 R2-B 460-490 500 520
    R2-B-WB-505 R2-B 450-490 510 520
    R2-B-WB-505 Blue (FITC) 450-490 505 520
    R2-B-WB-510 Blue (FITC) 450-490 510 515
    R3-B-NB-505 R3-B 470-490 505 520
    R4-B-NBBP-505 R4-B 460-495 505 510-560
    R4-B-WB-505 R4-B 470/40 505 535-50
    R4-DAPI-WB-395 R4-DAPI 365 395 445/50
    R4-DAPI-WB-415 UV (DAPI) 375/28 415 460/60
    R4-FITC-WB-495 R4-FITC 470/40 495 525/50
    R4-TRITC-WB-570 R4-TRITC 545/25 570 605/70
    R4-DRF-WB-595 R4-DRF 590/20 620 650
    R4-RHF-WB-500 R4-RHF 490/15 500 525/20
    R4-RHF-B-500 R4-RHF 460-490 500 525/20
    R4-UV-WB-395 R4-UV 390/40 415 460/50
    R4-UV-WB-395 UV (DAPI) 390/40 415 435-485
    R4-UV-WB-400 UV (DAPI) 330-385 400 420
    R4-UV-WB-415 UV (R4-UV) 375/30 415 460/50
    R4-UV-WB-420 UV (DAPI) 330-385 400 420-460
    R4-GFP-WB-495 R4-GFP 470/40 500 534/55
    R4-GFP-WB-500 R4-GFP 470/40 500 525/50
    R5-B-WB-505 R5-B 400-490 505 510-560
    R5-B-WB-505 Blue (FITC) 450-490 505 510
    R5-B-SB-505 R5-B 400-490nm 505 520
    R5-G-WB-565 Green 527-553 565 575
    R5-G-WB-575 Green (TRITC) 510-560 575 590
    R5-FITC-WB-495 Blue (FITC) 470/40 495 515/30
    R6-G-WB-575 Green (TRITC) 505-555 570 575
    R1-G-WB-570 Green (TRITC) 480-550 570 590
    R1-G-WB-590 Green (TRITC) 480-565 570 590
    R1-G-WB-570 R1-G 510-550 570 590
    R1-G-WB-575 R1-G 510-560 580 590
    R2-G-WB-570 R2-G 510-530 570 590
    R2-G-WB-575 R2-G 510-550 575 590
    R2-G-WB-575 Green (TRITC) 515-560 580 590
    R2-G-WB-575 Red (Cy5) 600-640 600 700
    R2-G-WB-590 Green (TRITC) 460-550 570 590
    R2-G-WB-595 Green (TRITC) 515-560 595 590
    R2-G-NBLP-546 R2-G 546/12 580 590
    R2-G-NBLP-575 R2-G 546/10 575 590
    R2-GFP-WB-505 Blue (FITC) 480/30 505 515
    R2-V-WB-430 R2-V 400-410nm 430 450
    R3-G-WB-575 R3-G 500-550 575 590
    R3-G-WBLP-575 R3-G 500-600 600 665
    R1-DAPI-WB-400 R1-DAPI 340-380 400 435-485
    R1-DAPI-WB-420 R1-DAPI(UV) 390/38 420 475-90
    R1-DAPI-WB-415 R1-DAPI 361-389 415 430-490
    R1-DP-WB-447 R1-DAPI 357/44 400 447/60
    R1-FITC-WB-500 R1-FITC 470/40 500 534/55
    R1-FITC-WB-505 R1-FITC 465-495 505 530/30
    R1-FITC-WB-510 R1-FITC 450-495 510 520
    R1-FITC-NB-505 R1-FITC 420-495 510 520
    R1-TRITC-WB-560 R1-TRITC (Green) 525/50 505 520-550
    R1-TRITC-WB-565 R1-TRITC 540/25 565 605/65
    R1-TRITC-WB-570 Green (TRITC) 546/82 570 590
    R1-TXRED-WB-560 R1-Texas Red 560/40 585 630/75
    R1-TRITC-NB-570 R1-TRITC 480-550 570 590
    R1-TXRED-WB-595 R1-Texas Red 560-595 595 600-660
    R1-TXRED-WB-600 R1-Texas Red 550/25 600 630/38
    R1-TXRED-WB-620 R1-Texas Red 585/20 620 624
    R1-GFP-WBLP-505 R1-GFP 480/40 505 510
    R1-GFP-WBBP-505 R1-GFP 480/40 505 535/50
    R1-GP-WB-524 R1-GFP 482/22 505 524/24
    R1-BFP-WB-420 R1-BFP 380/30 420 460/50
    R1-DAPI-NB-410 R1-DAPI 360-370 410 420-460
    R1-CFP-NB-450 R1-CFP 425-445 455 460-510
    R1-CFP-NB-455 R1-CFP 436/20 455 480/40
    R1-GFP-NB-490 R1-GFP 460-480 490 495-540
    R1-RP-NB-515 R1-RFP 490-500 515 515-560
    R1-YFP-NB-515 R1-YFP 500/20 515 535/30
    R1-YFP-NB-565 R1-YFP 535-555 565 570-625
    R1-MCH-NB-595 R1-mCherry 565-585 595 600-690
    R1-RP-WB-593 R1-RFP 542/20 570 593/40
    R1-BP-WB-610 R1-RFP 545-580 600 610
    R1-CY3-WB-560 R1-CY3 546/10 560 585/40
    R1-CY3-WB-565 R1-CY3 535/50 565 610/75
    R1-CY5-WB-660 R1-CY5 620/60 660 700/75
    R1-CY5-WB-655 R1-CY5 (RED) 620/50 655 690/50
    R1-CY7-WB-750 R1-CY7 710/75 750 810/90
    R2-FITC-NB-505 R2-FITC 470-495 500 510-550
    R2-TRITC-NB-570 R2-TRITC 540-550 570 575-625
    R2-CY5-WB-660 R2-CY5 621/60 660 700/75
    R3-CY5-WB-660 R2-CY5 640/30 660 690/50
    R4-CY3-WB-570 R4-CY3 545/25 570 605/70
    R5-CY5-WB-660 R5-CY5 630/60 660 700/75
    R5-UV-WB-415 UV (DAPI) 362-396 415 432-482
    L1DAPI L1-DAPI-WB-455 355-425 455 LP 470
    L1FITC L1-DAPI-WB-495 470/40 495 525/50
    L1TRITC L1-DAPI-WB-560 546/10 560 585/40
    LA1DAPI LA1-DAPI-WB-455 330-380 455 LP 470
    LA1FITC LA1-FITC-WB-495 450-480 500 515
    LA1TRITC LA1-TRITC-WB-560 510-550 570 590
    FURA-2 (Dual Band Filter) FURA-2 380 500 510
    LZ1-UV-WB-405 LZ1-UV 385/30 405 425/29
    LZ1-B-WB-493 LZ1-B 469/30 493 514/31
    LZ1-B-WB-500 LZ1-B 475/40 500 534/55
    LZ1-B-WB-505 Blue (FITC) 475/30 505 530/40
    LZ1-G-WB-575 LZ1-G 555/30 575 632/100
    R2-YFP-NB-600 Yellow (R2-YFP) 560/40 600 610
    R2-TRITC Green (TRITC) 527-555 565 577-633
    R2-TRITC-WB-565 Green (TRITC) 540/25 565 575
    R1-MCH-WB-590 mCherry 550/55 590 630/60
    C1R4-FITC-WB-495 Blue (FITC) 470/40 495 515
    C1R4-FITC-WB-525 Blue (FITC) 470/40 495 525/50
    C1R4-TRITC-WB-570 Green (TRITC) 540/25 565 605/55
    C1R1-UV-WB-410 UV (DAPI) 350/50 400 460/50
    R9-B-WB-470 Blue (FITC) 470-490 500 520
    R9-G-WB-530 Green (TRITC) 530-550 575 590
    R9-UV-WB-385 UV (DAPI) 330-385 400 460
    Blue (FITC) 470-495 505 510-550
    Green (TRITC) 540-550 570 575-625
    UV (DAPI) 360-370 410 420-460
    Red (CY5) 590–650 670 665-715
    FITC 475 500 530/15
    TRITC 545/20 562 605/40
    DAPI 365 405 445/15
    R1-DA-FI-NB R1-DA-FI DAPI/FITC
    R1-FI-TR-NB R1-FI-TR FITC/TRITC
    R1-FI-TX-NB R1-FI-TX FITC/Texas Red
    R1-DA-FI-TR-NB R1-DA-FI-TR DAPI/FITC/TRITC
    R1-DA-FI-TX-NB R1-DA-FI-TX DAPI/FITC/Texas Red

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